Calcium imaging

Calcium imaging is a microscopy technique to optically measure the calcium (Ca2+) status of an isolated cell, tissue or medium. Calcium imaging takes advantage of calcium indicators, fluorescent molecules that respond to the binding of Ca2+ ions by fluorescence properties. Two main classes of calcium indicators exist: chemical indicators and genetically encoded calcium indicators (GECI).[1] This technique has allowed studies of calcium signalling in a wide variety of cell types, and can be used to measure electrical activity in hundreds of neurons in cell culture, or in living animals during ongoing behavior.[2]

  1. ^ de Melo Reis, Ricardo Augusto; Freitas, Hércules Rezende; de Mello, Fernando Garcia (2020). "Cell Calcium Imaging as a Reliable Method to Study Neuron–Glial Circuits". Frontiers in Neuroscience. 14: 975. doi:10.3389/fnins.2020.569361. ISSN 1662-453X. PMC 7566175. PMID 33122991.
  2. ^ Siciliano, Cody A.; Tye, Kay M. (February 2019). "Leveraging calcium imaging to illuminate circuit dysfunction in addiction". Alcohol (Fayetteville, N.Y.). 74: 47–63. doi:10.1016/j.alcohol.2018.05.013. ISSN 1873-6823. PMC 7575247. PMID 30470589.

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